The video explores the process of hearing multiple sources of audio and how the human ear perceives them as individual sounds. It explains how the ear can process and isolate different waves from various sources, despite having only one eardrum per ear. The animation also discusses how the brain uses the frequency, amplitude, and temporal nature of sound to distinguish between multiple sources of audio. Additionally, the video raises the question of what happens when two sounds of similar frequency are playing at the same time. It provides an engaging and informative overview of the science behind sound mixing and how our brains interpret multiple sources of audio.